The discovery platforms of TheraMabs include advanced hybridoma technology and phage display technology, which offers full spectrum of discovery services for generation, characterization and selection of monoclonal antibodies against multiple targets, to help customers identify candidate antibody in the field of various diseases.
The service is as follows:
Sample production
Analytical Method Development
Antibody Generation
Antibody screening
Antibodies Optimization
discovery services process
Sample production
TheraMabs use CHO cells to produce high-quality monoclonal antibodies, fusion protein and other recombinant proteins for various experimental studies, which can satisfy the demand of early stage drug candidates, The instantaneous expression materiaks of 200 mg/L can produce enough protein for early drug screening, detection methods of development, the structure analysis and other research purposes.
Analytical Method Development
TheraMabs has successfully developed hundreds of assays, which can be used in the analysis of stoste, semi-finished products and finished products, including quantity(protein concentration by UV280), biological activity (Cell-based or binding ELISA assays), physical and chemical properties (purity, polymers, charge, isoelectric point, appearance, pH, osmolality), impurities (host cell protein, protein A, DNA residues, endotoxin, sterility), biochemical and biophysical characterization (intact mass of protein under reducing and non-reducing conditions, the primary sequence, peptide mapping, Glycan profiling).
Antibody Generation
TheraMabs has two advanced platforms for hybridoma technology and phage display technology, which can be isolated or combined to find idealized therapeutic antibodies.
Hybridoma technology platform
The hybridoma technology platform of TheraMabs have professional technology, advanced equipment, relying on the unique immune method and the combination of advanced technology, through every 1000 B cells can obtain hybridoma at 10 or so, the advantage of integration with high efficiency and high drop degree.
Phage display platform
TheraMabs offers natural antibodies, immune mice, humanized library transgenic mice, rats immune library, which provide support for library construction and validation, high-throughput screening, antibody sequencing, structure analysis, alteration and other services according to customer demand.
Antibody screening
TheraMabs provides high-throughput screening by enzyme-linked immunoassay, flow cytometry and fluorescence imaging-based binding methodologies, which usually adopt two rounds of selection, the first round of screening more than 10000 cell lines, the second round of screening more than 1000 cell lines, Detection and evaluation of the titer, purity, structure, glycosylation, and polymer of the obtained antibody were performed to ensure that the candidate antibody could be better used for functional analysis and animal experiment.
Antibodies Optimization
TheraMabs can provide antibody optimization services such as humanization and affinity maturation of monoclonal antibodies, also provide single service including the hybridoma cell lines sequencing, data of the construction of the library, high-throughput screening, fixed point mutation to optimize the Fc and the renovation of the glycosylation sites to improve the stability of the antibody and enhanced ADCC effect, etc.
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